How Kalvatin works: the science behind the healing.


A revolutionary discovery

The Kalvatin story begins in the 1970s. Icelandic scientists, found that staff at Scandinavian fish processing facilities had unusually soft and supple hands—unexpected, given that the work should leave hands rather cracked and sore. Was there something in the fish that accelerated healing? The answer was yes. For a long time, it was thought to be fish oil, but research demonstrated that in specific marine organisms there is a cold-adapted enzyme with unique healing properties.1,2

When that enzyme comes in contact with soft tissue and mucous membranes at body temperature, it becomes super active, forming an effective layer of protection against viruses and bacteria while cleaving necrotic tissue, reducing inflamation and helping to accelerate the healing process. This development eventually led to a worldwide patent on the marine enzyme (cold-adapted trypsin).


Natural marine origin

Kalvatin is a naturally effective, sustainable product. Its active ingredient, cold-adapted trypsin, is extracted naturally from North Atlantic cod in the deep and icy waters of the North Sea. The enzyme is produced in the pancreas of the cod, but is secreted into the intestines. Therefore, the extraction is a byproduct of fish processing and creates no waste or damage to the marine ecosystem.


Safe for your pets

The unique enzyme in Kalvatin has been widely tested and is approved for human use in Europe. The natural enzyme maintains a high safety profile and has proven in clinical studies to be both safe and highly effective. Safety and toxicology has been documented with more than 3.5 million doses administered. All of the ingredients in Kalvatin are on this list of approved ingredients by AAFCO and are GRAS (generally regarded as safe) by the FDA. We utilize GMP facilities and hold ourselves to extremely stringent quality guidelines.  Clinical trials for both our dental and dermal applications have been extensively tested on animals in the United States. The results have demonstrated not only their safety, but also Kalvatin’s potential as an effective product proven to help reduce microbial adhesion, cleave necrotic tissue, disarm viruses, and act as a soft-tissue healing agent. 


Active enzymatic barrier

Together with a hypertonic solution, the enzyme forms a temporary barrier on skin or mucosa to help strengthening the defense against infections. The barrier reduces the ability of viruses and bacteria to attach to cells and cause disease.  The enzyme's normal activity level is at 4°C. On contact with body temperature the enzyme becomes superactive and the catalytic activity is increased. The catalytic activity is 40-50 times higher than the corresponding enzyme in mammals. 

  • Isolated from Atlantic cod
  • Cold adapted protease24kDa
  • Serine peptidase
  • Crystal structure (PDB ID2EEK) 
  • High specific activity, low KM, high kcat
  • Sensitive to inactivation by heat, low pHand autolysis

When bacteria colonize an area—in a pet’s mouth, or on a hotspot, for instance—they attach to surface proteins. The bacteria secrete biofilm, a “glue” containing proteins and starches, which works as a cover, protecting the bacteria. The unique features of the trypsin in Kalvatin make it super-active at about 37°C (around normal body temperature for cats and dogs), allowing it to prevent bacteria from causing infection or forming plaque and tartar.

1. Gudmundsdóttir, A., & Pálsdóttir, HM (2005). Marine Biotechnology (New York, NY), 7 (2), 77-88.
2. Mangion, M. (2004). Evaluation of an enzyme extracted from fish for topical use in the treatment of pressure sores., 1–1.
Trypsin (Cold-adapted marine enzyme) 0.004%